We have implemented methods to process artificial materials and polymers using histotechnology. Most studies are performed with resin-based method, but also paraffing and cryo methods are used. Titan screws and other unsectionable materials are processed into resin and the sections are made with grinding method.
Toxicity and xenograft studies for drug development
We are eager to help our clients to conduct toxicity studies and calculate optimal tissue and slide amount for the study. As we compress information by using multi-tissue blocks and multi-tissue sections, we can decrease the amount of slides significantly without losing information. This saves time and money, including provides a faster, as well as more accurate histopathological interpretation service.
One of BioSiteHisto’s strengths is immunohistochemical studies of xenograft tissues. We have an excellent selection of antibodies for xenograft studies targeted against human and mouse cells.
We have a variety of options to study drug efficacy, oncological effects, toxicity and host specific immunological reactions utilizing different markers from xenograft or allograft tissues.
An example of our selection of immunohistochemical services for xenograft tissues:
- Proliferation and mitosis (Ki67, PCNA, PHH3 etc.)
- Apoptosis (Tunel, Caspase3, Cytodeath M30 etc.)
- Tumor necrosis (analysis using HE stained slides)
- Hypoxia (CA9, HIF1, GLUT1 etc.)
- Angiogenesis and microvessel density (CD31, CD34 etc.)
- Neuronal markers (GFAP, MBP, IBA1, SYP etc.)
- Specific cancer markers
- Innate immunity of the host (CD3, CD4, F4/80 etc.)
- Signaling pathways
- Treatment response markers
![CKpan [BS5] – Xenograft, human carcinoma cells in mouse tissue](https://www.biositehisto.com/wp-content/uploads/ckpan-bs5-xenograft-human-carcinoma-cells-in-mouse-tissue-370x278.jpg){kind=link}