Compared to fluorescence and biotin based immunodetections, immunoperoxidase staining is more sensitive, permanent and offers valuable information on tissue morphology. At BioSiteHisto, immunostainings can be performed using permanent chromogens like DAB, permanent red and permanent green.
A wide selection of optimized protocols for human, xenograft and different animal tissues have been optimized using multi-tissue sections. BioSiteHisto’s excellent results in external quality assessments of IHC is a true testament of our standard and GLP compliance. We use Labvision Autostainer immunostainer, which allows staining of a wide range of immunoreagents or even your own reagents if needed.
Immunoperoxidase staining with HRP (horse radish peroxidase) and DAB visualization offers great possibilities to study target antigens from tissue sections (Paraffin section & frozen sections) with high sensitivity and contrast.
In special cases, when pigmentation and/or pigmented cells do not allow the interpretation of DAB visualized slides, alkalinephosphatase based immunostainings can offer extra value. Red chromogen (Permanent red) signal is easily distinguished from pigmented cells for eg. skin tissue and melanoma diagnostic. In addition, Permanent red also offers great fluorescence value as it is possible to detect in bright field and dark field microscopy.
Contact us today to get more information on how we can help you!
